Massively Parallel Digital Transcriptional Profiling Of Single Cells

CUT&Tag for efficient epigenomic profiling of small samples and single ….

Apr 29, 2019 . The advent of massively parallel sequencing and the dramatic reduction in cost per base has fueled a genomics revolution, however, the full promise of epigenomic profiling has lagged owing to ....

https://www.nature.com/articles/s41467-019-09982-5.

Scalable, multimodal profiling of chromatin accessibility, gene ....

Jun 03, 2021 . Recent technological advances have enabled massively parallel chromatin profiling with scATAC-seq (single-cell assay for transposase accessible chromatin by sequencing). Here we present ATAC with ....

https://www.nature.com/articles/s41587-021-00927-2.

Overlapping transcriptional programs promote survival and ….

Jun 28, 2022 . The second intervention was to deliver CNTF in parallel with Pten deletion. ... approximately 8,000-12,000 single cells were loaded on each channel and approximately 3,000-7,000 cells were recovered. Libraries were sequenced on Illumina HiSeq 2500, or NovaSeq platforms (Paired end reads: Read 1, 26 bases, Read 2, 98 bases). ... The single-cell ....

https://www.sciencedirect.com/science/article/pii/S0896627322005402.

Plasticity of Epididymal Adipose Tissue in Response to Diet ….

Feb 02, 2021 . Deconstructing adipogenesis induced by beta3-adrenergic receptor activation with single-cell expression profiling. Cell Metab, 28 (2018), pp. 300-309.e4. ... RNA velocity of single cells. Nature, 560 (2018), pp. 494-498. ... Simultaneous transcriptional and epigenomic profiling from specific cell types within heterogeneous tissues in vivo. Cell ....

https://www.sciencedirect.com/science/article/pii/S1550413120306598.

GEO Accession viewer - National Center for Biotechnology Information.

Mar 27, 2019 . NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data..

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL11154.

Single-cell profiling of the developing mouse brain and spinal ….

To facilitate scalable profiling of single cells, we developed split-pool ligation-based transcriptome sequencing (SPLiT-seq), ... J. P. Radich, T. S. Mikkelsen, B. J. Hindson, J. H. Bielas, Massively parallel digital transcriptional profiling of single cells. ....

https://www.science.org/doi/10.1126/science.aam8999.

Overlapping transcriptional programs promote survival and.

Jun 28, 2022 . Several interventions promote survival and regeneration of retinal ganglion cells following injury. scRNA-seq analysis shows that these interventions downregulate a gene expression program associated with cell death and upregulate programs associated with survival and regeneration. Overexpression of some regeneration module genes enhances RGC survival ....

https://www.cell.com/neuron/fulltext/S0896-6273(22)00540-2.

Landscape and Dynamics of Single Immune Cells in ….

To characterize the immune cells in HCC, we applied scRNA-seq methods to study CD45 + cells isolated from tumors and four immune-relevant sites (adjacent liver, hepatic LNs, blood, and ascites) of 16 treatment-naive liver cancer patients (Figures S1A-S1C; Tables S1 and S2).For patient DSN09, both 10x Genomics and SMART-seq2 methods were applied in parallel, giving ....

https://www.cell.com/cell/fulltext/S0092-8674(19)31119-5.

Loupe Browser Tutorial -Software -Single Cell Gene Expression.

This set of tutorials review the major analysis capabilities Loupe Browser provides for analyzing 3' Single Cell Gene Expression, Feature Barcode data, and 5' V(D)J and Single Cell Gene Expression data. ... You should see a screen with the t-SNE projection for the cells from the AML dataset. ... Massively parallel digital transcriptional ....

https://support.10xgenomics.com/single-cell-gene-expression/software/visualization/latest/tutorial.

Distinct functions of three chromatin remodelers in activator ….

Jul 06, 2022 . Author summary ATP-dependent chromatin remodelers (CRs), including SWI/SNF and RSC in budding yeast, are thought to stimulate transcription by repositioning or evicting promoter nucleosomes, and we recently implicated the CR Ino80C in this process as well. The relative importance of these CRs in stimulating activator binding and recruitment of TATA ....

https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010277.